12 Top startups in Brookline for April 2025

We rank Preply, XRHealth and C14 in Brookline along with 9 other startups based on an algorithm that scores startups based on total investment, employee count, and quarterly website traffic. Brookline is part of Boston Area startup ecosystem which ranks #5 globally and #4 in North America.

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Accelerators

Top Unicorn Startups in Brookline for 2025

airSlate is the only unicorn in Brookline raising more than USD 1.25B. It is the undefined ranked unicorn in North America.
SB Score:564

Empowering teams to innovate, automate, and digitally transform business operations. Our solutions drive creativity and efficiency, revolutionizing the way organizations work.

Industry:
Software & Data
Subindustry:
Software

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Total amount:1

Best Startups in Brookline for 2025

SB Score:715

Preply connects learners with tutors globally for personalized language lessons online. Students can create custom learning plans and choose their own schedule for flexibility.

Industry:
Edtech
Subindustry:
Edtech

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SB Score:472

XRHealth offers a VR platform for remote treatment, revolutionizing telemedicine. Users can access virtual therapy sessions from the comfort of their own homes, making healthcare more accessible and convenient.

Industry:
Healthtech
Subindustry:
Health Care

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3rd

C14
SB Score:295

C14 offers a user-friendly payment widget for websites and dApps, compatible with various blockchain ecosystems. It simplifies transactions and enhances the online shopping experience with seamless integration.

Industry:
Fintech
Subindustry:
Blockchain

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SB Score:281

Panoptic is a perpetual, oracle-free, instant-settlement options trading protocol on the Ethereum blockchain. Panoptic enables the permissionless trading of options on top of any asset pool in the Uniswap v3 ecosystem and seeks to develop a trustless, permissionless, and composable options product, i.e., do for decentralized options markets what x*y=k automated market maker protocols did for spot trading. Offering advanced solutions for trading, liquidity provision, and risk management, the company caters to various strategies and roles in the DeFi ecosystem.

Industry:
Fintech
Subindustry:
Blockchain

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5th

ViRB
SB Score:273

ViRB sources, trains, and deploys clinical research professionals for the life sciences industry using technology. Get started with ViRB to access a pool of skilled professionals ready to contribute to your projects.

Industry:
Software & Data
Subindustry:
Enterprise Technology

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SB Score:266

Edgewood Oncology is a clinical-stage biotechnology company dedicated to unlocking the potential of BTX-A51 for patients with genetically targeted solid tumors and hematologic malignancies. Their innovative approach aims to provide new treatment options and improve outcomes for those affected by these challenging conditions.

Industry:
Healthtech
Subindustry:
Biotechnology

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SB Score:240

Layer Health focuses on resolving the healthcare information problem by utilizing artificial intelligence to execute any clinical, administrative, or research work that involves chart review from unstructured data.

Industry:
Healthtech
Subindustry:
Health Care

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SB Score:220

Revolutionizing building access, our smartphone video intercom system enhances tenant satisfaction in multi-tenant buildings. Streamlined control and ease of use boost retention for building managers, ensuring seamless access for all users.

Industry:
Hardware & IoT
Subindustry:
Internet of Everything

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SB Score:171

EatWell partners with health insurers to provide Food is Medicine meal kits designed to combat food insecurity and lower healthcare costs for diabetic patients. By prescribing nutritious meals, EatWell aims to enhance health outcomes and promote well-being within vulnerable populations.

Industry:
Foodtech
Subindustry:
Food and Beverage

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SB Score:71

Exaloop develops user-friendly computational tools with high-performance capabilities, specializing in bridging the gap between technology and life sciences. Their focus on bioinformatics ensures efficient and effective solutions for various industries.

Industry:
Edtech
Subindustry:
Education-Other

National Rank (United States)

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Total amount:12

Top 4 Healthtech Startups in Brookline for 2025

The top three Healthtech startups in Brookline have raised more than USD 132M. Brookline is part of Boston Area startup ecosystem which ranks #2 globally for the Healthtech industry.
SB Score:472

XRHealth offers a VR platform for remote treatment, revolutionizing telemedicine. Users can access virtual therapy sessions from the comfort of their own homes, making healthcare more accessible and convenient.

Industry:
Healthtech
Subindustry:
Health Care

National Rank (United States)

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Regional Rank (North America)

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Global Rank (WorldWide)

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SB Score:266

Edgewood Oncology is a clinical-stage biotechnology company dedicated to unlocking the potential of BTX-A51 for patients with genetically targeted solid tumors and hematologic malignancies. Their innovative approach aims to provide new treatment options and improve outcomes for those affected by these challenging conditions.

Industry:
Healthtech
Subindustry:
Biotechnology

National Rank (United States)

0

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Regional Rank (North America)

0

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Global Rank (WorldWide)

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SB Score:240

Layer Health focuses on resolving the healthcare information problem by utilizing artificial intelligence to execute any clinical, administrative, or research work that involves chart review from unstructured data.

Industry:
Healthtech
Subindustry:
Health Care

National Rank (United States)

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Regional Rank (North America)

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Total amount:4

Top 1 Software & Data Startups in Brookline for 2025

The top three Software & Data startups in Brookline have raised more than USD 5M. Brookline is part of Boston Area startup ecosystem which ranks #5 globally for the Software & Data industry.

1st

ViRB
SB Score:273

ViRB sources, trains, and deploys clinical research professionals for the life sciences industry using technology. Get started with ViRB to access a pool of skilled professionals ready to contribute to your projects.

Industry:
Software & Data
Subindustry:
Enterprise Technology

National Rank (United States)

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Regional Rank (North America)

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Total amount:1

Top 1 Ecommerce & Retail Startups in Brookline for 2025

Brookline is part of Boston Area startup ecosystem which ranks #11 globally for the Ecommerce & Retail industry.
Unranked

Busy Beauty helps women reclaim time by offering efficient personal care solutions. The startup streamlines routines, providing products that simplify and speed up daily beauty tasks.

Industry:
Ecommerce & Retail
Subindustry:
Consumer Goods

National Rank (United States)

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Total amount:1

Most Funded Startups in Brookline for 2025

The top three most funded startups in Brookline have raised total funding of more than USD 306M. Preply has 0.63% more funding than XRHealth.
SB Score:715

Preply connects learners with tutors globally for personalized language lessons online. Students can create custom learning plans and choose their own schedule for flexibility.

Industry:
Edtech
Subindustry:
Edtech

National Rank (United States)

0

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Regional Rank (North America)

0

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Global Rank (WorldWide)

0

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SB Score:472

XRHealth offers a VR platform for remote treatment, revolutionizing telemedicine. Users can access virtual therapy sessions from the comfort of their own homes, making healthcare more accessible and convenient.

Industry:
Healthtech
Subindustry:
Health Care

National Rank (United States)

0

View

Regional Rank (North America)

0

View

Global Rank (WorldWide)

0

Browse
View full page
SB Score:266

Edgewood Oncology is a clinical-stage biotechnology company dedicated to unlocking the potential of BTX-A51 for patients with genetically targeted solid tumors and hematologic malignancies. Their innovative approach aims to provide new treatment options and improve outcomes for those affected by these challenging conditions.

Industry:
Healthtech
Subindustry:
Biotechnology

National Rank (United States)

0

View

Regional Rank (North America)

0

View

Global Rank (WorldWide)

0

Browse
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Total amount:12

Top Hiring Startups in Brookline by Employee Number

The top three hiring startups in Brookline have an median employee size that is between [101-250]
SB Score:715

Preply connects learners with tutors globally for personalized language lessons online. Students can create custom learning plans and choose their own schedule for flexibility.

Industry:
Edtech
Subindustry:
Edtech

National Rank (United States)

0

View

Regional Rank (North America)

0

View

Global Rank (WorldWide)

0

Browse
View full page
SB Score:472

XRHealth offers a VR platform for remote treatment, revolutionizing telemedicine. Users can access virtual therapy sessions from the comfort of their own homes, making healthcare more accessible and convenient.

Industry:
Healthtech
Subindustry:
Health Care

National Rank (United States)

0

View

Regional Rank (North America)

0

View

Global Rank (WorldWide)

0

Browse
View full page

3rd

ViRB
SB Score:273

ViRB sources, trains, and deploys clinical research professionals for the life sciences industry using technology. Get started with ViRB to access a pool of skilled professionals ready to contribute to your projects.

Industry:
Software & Data
Subindustry:
Enterprise Technology

National Rank (United States)

0

View

Regional Rank (North America)

0

View

Global Rank (WorldWide)

0

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4th

C14
SB Score:295

C14 offers a user-friendly payment widget for websites and dApps, compatible with various blockchain ecosystems. It simplifies transactions and enhances the online shopping experience with seamless integration.

Industry:
Fintech
Subindustry:
Blockchain

National Rank (United States)

0

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Regional Rank (North America)

0

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Global Rank (WorldWide)

0

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Total amount:12

Explore the Startup Ecosystem of Brookline

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Top Cities for Startups in United States - Ranked by StartupBlink

StartupBlink ranks the startup ecosystems of more than 100 countries and 1,000 cities. Download our latest Global Startup Ecosystem Report.
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2024 National Rank CityGlobal Rank
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Total Score

Access subscores for 1000 cities and 100 countries

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Quantity Score
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Quality Score
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Business Score
1st
0
1st
0
710.966
00.00
00.00
00.00
2nd
0
2nd
0
251.377
00.00
00.00
00.00
3rd
0
4th
0
121.937
00.00
00.00
00.00
4th
0
5th
0
109.679
00.00
00.00
00.00
5th
0
12th
0
52.052
00.00
00.00
00.00
6th
0
15th
0
42.256
00.00
00.00
00.00
7th
+
1
17th
+
4
41.259
00.00
00.00
00.00
8th
-
1
19th
-
1
37.971
00.00
00.00
00.00
9th
0
24th
-
2
34.098
00.00
00.00
00.00
10th
0
25th
+
1
33.768
00.00
00.00
00.00
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